4t1 metastatic mice breast tumor cell line  (ATCC)

Journal: Pharmaceutics

Article Title: In Vitro and Preclinical Antitumor Evaluation of Doxorubicin Liposomes Coated with a Cholesterol-Based Trimeric β-D-Glucopyranosyltriazole

doi: 10.3390/pharmaceutics15122751

Figure Lengend Snippet: Half-maximum inhibitory concentration (IC 50 ) for Free-DOX, GlcL-DOX, and PegL-DOX against 4T1 tumor cells ( p < 0.05).

Article Snippet: In this study, the 4T1 metastatic mice breast tumor cell line (CRL-2539–ATCC) was grown in RPMI 1640 medium, supplemented with 10% ( v / v ) of FBS, penicillin (100 IU/mL), and streptomycin (100 μg/mL).

Techniques: Concentration Assay

Journal: Pharmaceutics

Article Title: In Vitro and Preclinical Antitumor Evaluation of Doxorubicin Liposomes Coated with a Cholesterol-Based Trimeric β-D-Glucopyranosyltriazole

doi: 10.3390/pharmaceutics15122751

Figure Lengend Snippet: Flow cytometry panels obtained from 4T1 cells stained with Annexin-V-Alexa Fluor-488 and PI solution (PE-Texas Red-A channel) constituted by the: ( A ) Negative Control group; ( B ) cells treated with Free-DOX; and ( C , D ) cells treated with GlcL-DOX and PegL-DOX formulations, respectively.

Article Snippet: In this study, the 4T1 metastatic mice breast tumor cell line (CRL-2539–ATCC) was grown in RPMI 1640 medium, supplemented with 10% ( v / v ) of FBS, penicillin (100 IU/mL), and streptomycin (100 μg/mL).

Techniques: Flow Cytometry, Staining, Negative Control

Journal: Pharmaceutics

Article Title: In Vitro and Preclinical Antitumor Evaluation of Doxorubicin Liposomes Coated with a Cholesterol-Based Trimeric β-D-Glucopyranosyltriazole

doi: 10.3390/pharmaceutics15122751

Figure Lengend Snippet: Antitumor activity of Saline (Control), Free-DOX, GlcL-DOX, and PegL-DOX against a 4T1 breast tumor implanted in the flank of female BALB/c mice. Each treatment was intravenously administered four times, every 2 days, at a dose of 5 mg/kg. Results are presented as the mean ± SD ( n = 7). All data were analyzed by one-way ANOVA analysis of variance, followed by Tukey’s post-test. * represents statistical differences between GlcL-DOX and PegL-DOX formulations.

Article Snippet: In this study, the 4T1 metastatic mice breast tumor cell line (CRL-2539–ATCC) was grown in RPMI 1640 medium, supplemented with 10% ( v / v ) of FBS, penicillin (100 IU/mL), and streptomycin (100 μg/mL).

Techniques: Activity Assay, Saline, Control

Journal: Pharmaceutics

Article Title: In Vitro and Preclinical Antitumor Evaluation of Doxorubicin Liposomes Coated with a Cholesterol-Based Trimeric β-D-Glucopyranosyltriazole

doi: 10.3390/pharmaceutics15122751

Figure Lengend Snippet: Body weight variation of 4T1 tumor-bearing female mice after treatment with Saline 0.9% (Control group), Free-DOX, PegL-DOX, or GlcL-DOX. Results are presented as the mean ± SD ( n = 7). All data were analyzed by one-way ANOVA analysis of variance, followed by Tukey’s post-test. * represents statistical differences ( p < 0.05) between the liposome-treated groups and Free-DOX group.

Article Snippet: In this study, the 4T1 metastatic mice breast tumor cell line (CRL-2539–ATCC) was grown in RPMI 1640 medium, supplemented with 10% ( v / v ) of FBS, penicillin (100 IU/mL), and streptomycin (100 μg/mL).

Techniques: Saline, Control

Journal: Pharmaceutics

Article Title: In Vitro and Preclinical Antitumor Evaluation of Doxorubicin Liposomes Coated with a Cholesterol-Based Trimeric β-D-Glucopyranosyltriazole

doi: 10.3390/pharmaceutics15122751

Figure Lengend Snippet: Biochemical analyses of 4T1 tumor-bearing female mice after treatment with Saline 0.9% (control group), DOX-free, PegL-DOX, or GlcL-DOX.

Article Snippet: In this study, the 4T1 metastatic mice breast tumor cell line (CRL-2539–ATCC) was grown in RPMI 1640 medium, supplemented with 10% ( v / v ) of FBS, penicillin (100 IU/mL), and streptomycin (100 μg/mL).

Techniques: Saline, Control